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P5475

Sigma-Aldrich

C-CMV-24 Sequencing Primer

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About This Item

MDL number:
MFCD01634562
UNSPSC Code:
12352200

grade

for molecular biology

shipped in

dry ice

storage temp.

−20°C

General description

The C-CMV-24 sequencing primer is a chemically synthesized, 24 base, single strand oligodeoxyribonucleotide designed for dsDNA or ssDNA sequencing of FLAG® fusion proteins at the N-terminus of the pFLAG-CMV expression vectors. The primer is complementary to map position nucleotides 1116-1139 of negative, non-coding DNA strand of the pFLAG-CMV-1 vector and nucleotides 1073-1096 of negative, non-coding DNA strand of the pFLAG-CMV-2 vector.

Application

The N-CMV-30 sequencing primer is a chemically synthesized, 30 base, single strand oligodeoxyribonucleotide designed for dsDNA or ssDNA sequencing of FLAG® fusion proteins at the C-terminus of the pFLAG-CMV expression vectors. The primer is complementary to map position nucleotides 818-847 of positive, coding DNA strand of the pFLAG-CMV vectors.

Components

The primer is supplied at an initial concentration of 5 uM in 1xTE buffer. The nucleotide sequence is as follows: 5′-TAT-TAG-GAC-AAG-GCT-GGT-GGG-CAC-3′

Other Notes

(5′-TAT TAG GAC AAG GCT GGT GGG CAC-3′)

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P5475-BULK:
P5475-1UG:
P5475-1UG-KC:
P5475-VAR:
P5475PROC:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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