Skip to Content
Merck
All Photos(1)

Documents

Safety Information

P0059

Sigma-Aldrich

Pro-carboxypeptidase B human

≥2.0 units/mg protein

Synonym(s):

Thrombin Activatable Fibrinolysis Inhibitor (TAFI)

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD02689174
UNSPSC Code:
12352202
NACRES:
NA.77

Quality Level

200

specific activity

≥2.0 units/mg protein

mol wt

60 kDa

UniProt accession no.

P15086

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CPB1(1360)

Biochem/physiol Actions

TAFI is supplied in its 60 kDa zymogen form. The N-terminal of TAFI contains four N-linked glycans and a plasminogen recognition sequence. Hydrolysis of the activation peptide by thrombin releases a 36-kDa active proteolytic domain homologous to tissue carboxypeptidase B. Complexation with thrombomodulin increases thrombin activation of TAFI 1250 fold. TAFI exerts it′s inhibition of fibrinolytic activity by binding to plasminogen and by cleaving C-terminal lysine residues of fibrin resulting in the reduction of plasmin and tPA binding sites on fibrin. Experiments in TAFI-deficient mice point to a regulatory role on complement C5a.

Unit Definition

One unit will hydrolyze 1 micromole of Hippuryl-L-Arginine per minute at pH 7.4 at 25 °C.

Physical form

Solution containing 20 mM Hepes and 150 mM Sodium chloride. pH 7.4

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P0059-50UG:
P0059-BULK:
P0059-VAR:
P0059-50UG-PW:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Toshihiko Nishimura et al.
Blood, 109(5), 1992-1997 (2006-11-16)
Plasma procarboxypeptidase B (proCPB) is activated by the endothelial thrombin-thrombomodulin [corrected] complex. Activated proCPB [corrected] (CPB) functions as a fibrinolysis inhibitor, but it may play a broader role by inactivating inflammatory mediators. To test this hypothesis, C5a-induced alveolitis was studied
B N Bouma et al.
Journal of thrombosis and haemostasis : JTH, 1(7), 1566-1574 (2003-07-23)
Recently, a new inhibitor of fibrinolysis was described, which downregulated fibrinolysis after it was activated by thrombin, and was therefore named TAFI (thrombin-activatable fibrinolysis inhibitor; EC 3.4.17.20). TAFI turned out to be identical to the previously described proteins, procarboxypeptidase U

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service