MAK512

Malate Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

• UNSPSC Code: 12352204
• NACRES: NA.84

Properties

• Quality Level: 100
• detection method: colorimetric
• relevant disease(s): Alzheimer′s disease; neurological disorders; neurodegenerative disorders
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Malate dehydrogenase (MDH) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 is found in the cytoplasm and plays a key part in the malateaspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer′s disease.

Features and Benefits

• Linear detection range: 0.5 to 65 U/L
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for malate dehydrogenase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)

Principle

The Malate Dehydrogenase Assay Kit is a non-radioactive, colorimetric MDH assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Safety Information

• Pictograms: GHS02,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H290,H315,H319,H336
• Precautionary Statements: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3