MAK299

NNMT Inhibitor Screening Assay Kit

sufficient for 100 fluorometric tests

• UNSPSC Code: 12352200
• NACRES: NA.84

Properties

• detection method: fluorometric
• relevant disease(s): endocrinological disorders, diabetes; obesity; cancer; neurological disorders
• storage temp.: −70°C

Description

General description

N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy.

Suitability

Suitable for screening/studying/characterizing potential inhibitors of N′-Nicotinamide Methyltransferase (NNMT).

Principle

This NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using the Thiol Detecting Probe, generating an enhanced fluorescence signal (λex = 392 nm/ λem = 482 nm). In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.

Safety Information

• Pictograms: GHS02,GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H226,H312,H317,H318,H334,H361d,H412
• Precautionary Statements: P210 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 3 - Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 3 - Flammable liquids
• Flash Point(F): 84.2 °F
• Flash Point(C): 29 °C