MAK255

Cysteine Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

• Synonym(s): Cysteine Quantitation Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• detection method: fluorometric
• relevant disease(s): cardiovascular diseases
• storage temp.: −20°C

Description

General description

Cysteine (CYS) is a non-essential, sulfur containing amino acid in humans, related to cystine. Cysteine is important for protein synthesis, detoxification, and diverse metabolic functions. It is found in beta-keratin, which comprises the main protein in nails, skin, and hair. Cysteine is also important in collagen production, as well as skin elasticity and texture. A component of the antioxidant, glutathione, cysteine plays a role in the metabolism of essential biochemicals such as coenzyme A, heparin, and biotin. An elevated level of total cysteine can predict cardiovascular disease and metabolic syndromes. Cysteine deficiency is known to be one of the consequences of aging.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of cysteine in serum, plasma, and other biological fluids.

Principle

The Cysteine Assay Kit detects the physiological concentration of cysteine in a variety of biological fluids. The assay is based on the cleavage of thiol group of reduced cysteine generating a fluorometric product (ex = 365 nm/ em = 450 nm), directly proportional to the amount of total cysteine in the sample. The assay is specific and other thiol-based amino acids do not interfere with the assay. This high-throughput adaptable assay kit is simple and sensitive enough to detect to 10 µM of cysteine in a variety of samples.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup