MAK186

Xanthine/Hypoxanthine Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

• Synonym(s): Xanthine and Hypoxanthine Detection Kit
• EC Number: 200-718-6
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• detection method: colorimetric; fluorometric
• relevant disease(s): neurological disorders; rheumatological diseases
• storage temp.: −20°C

Description

General description

Xanthine is a purine base present in body fluids, tissues, and plants cells. It is also found in urinary calculi. Xanthine is formed by the oxidation of hypoxanthine during the degradation of adenosine monophosphate (AMP) to uric acid. Xanthine/ hypoxanthine measurements in plasma and urine have been used to monitor allopurinol therapy. Cerebrospinal fluid (CSF) xanthine/hypoxanthine levels have been used as therapeutic guides and disease progression markers in hydrocephalus.

The Xanthine/Hypoxanthine Assay Kit is a highly sensitive assay for determining xanthine/hypoxanthine levels (ranging from 4-20 nmole/well for the colorimetric assay and 40-200 pmole/well for the fluorometric assay) in a variety of biological samples. Xanthine/hypoxanthine concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm) or fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the xanthine/hypoxanthine present.
Note: The fluorometric assay is ~10 times more sensitive than the colorimetric assay.

Features and Benefits

Compatible with high-throughput liquid handling systems.

Suitability

Suitable for the detection of Xanthine/Hypoxanthine in a variety of samples such as tissue, cells, and body fluids.

Principle

The Xanthine/Hypoxanthine Assay Kit is a highly sensitive assay for determining xanthine/hypoxanthine levels (ranging from 4-20 nmole/well for the colorimetric assay and 40-200 pmole/well for the fluorometric assay) in a variety of biological samples. Xanthine/hypoxanthine concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm) or fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the xanthine/hypoxanthine present.

Other Notes

The fluorometric assay is ~10 times more sensitive than the colorimetric assay.

Kit Components Only

• Xanthine Assay Buffer
• Peroxidase Substrate
• Xanthine Enzyme Mix
• Developer
• Xanthine Standard

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334,H412
• Precautionary Statements: P261 - P272 - P273 - P280 - P284 - P302 + P352
• Hazard Classifications: Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids