MAK176

Granzyme B Activity Assay Kit

Sufficient for 100 Fluorometric tests

• Synonym(s): CTLA1, GZMB Activity Assay Kit
• EC Number: 200-664-3
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 fluorometric tests
• detection method: fluorometric
• relevant disease(s): hematological disorder; immunological diseases; cancer
• storage temp.: −20°C
• Gene Information: human ... GZMB(3002) ; mouse ... GZMB(14939) ; rat ... GZMB(171528)

Description

General description

GZMB codes for granzyme B.[1]

Granzyme B (gzm B) is a peptidase that induces cytotoxic T lymphocyte (CTL)-mediated DNA fragmentation and apoptosis in allogeneic target cells.[2] It cleaves after aspartic acid residues in substrates. Granzyme B-mediated release of mitochondrial cell death factors are required for the activation of caspase-3. Furthermore, the degradation of granzyme B is known to decrease the susceptibility of cancer cells to NK-cell-mediated lysis during hypoxia. Granzyme B activity assays can be used for studying T cell immunity, CTL responses, cell-mediated cytotoxicity, and apoptosis.[3]
Gzm B is a pro-apoptotic member of the granzyme family.[4] It is a neutral serine protease with Aspase activity. Gzm B is expressed exclusively in the granules of activated cytolytic T cells, natural killer cells and lymphokine-activated killer cells.[2]

Application

• A Microfluidic Platform for Single Cell Fluorometric Granzyme B Profiling: Describes a technological advancement where the Granzyme B Activity Assay Kit is integrated into a microfluidic platform, enabling high-throughput and precise measurements of Granzyme B activity at the single-cell level, applicable in both research and clinical diagnostics (Briones et al., 2020).

Suitability

Suitable for the detection of granzyme B activity in biological samples and for the characterization of granzyme B inhibitors.

Principle

Granzyme B activity is determined using an enzyme-catalyzed reaction in which the non-fluorescent substrate, Ac-IEPD-AFC, is hydrolyzed resulting in the release of AFC (λex = 380/λem = 500 nm) proportional to the enzymatic activity present. One unit of granzyme B is the amount of enzyme that hydrolyzes 1 pmole of the substrate per minute at 37 °C.

Kit Components Only

• Granzyme B Assay Buffer
• Granzyme B Substrate
• Granzyme B Positive Control
• AFC Standard, 1 mM

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1