Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters.
Suitability
The Pyrophosphate Assay Kit provides a simple and direct flourescent microplate procedure for measuring pyrophosphate in a variety of samples.
Principle
The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =370/λem = 470 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.
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