MAK117

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

• Synonym(s): Glycerin Assay Kit, Glycerine Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 200 colorimetric or fluorometric tests
• application(s): cosmetics; environmental; food and beverages
• detection method: colorimetric; fluorometric
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Principle

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

Safety Information

• Storage Class Code: 10 - Combustible liquids