Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

Safety Information

MAK074

Sigma-Aldrich

Ascorbic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Vitamin C Assay Kit

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Suitability

Suitable for use with biological fluids, food samples, and growth media. Proteins present in the sample can interfere with this assay. Protein containing samples are best analyzed using the Ascorbic Acid Assay II (MAK075). Alternatively, samples can be deproteinized using a 10 kDa Molecular Weight Cut-Off Spin Filter.

Principle

In this assay, Ascorbic Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/ λem = 587 nm) product, proportional to the ascorbic acid present.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H319,H334

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

Please refer to KIT Component information

FSL

Please refer to KIT Component information

ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

Please refer to KIT Component information

Cartagena Act

Please refer to KIT Component information

JAN Code

キットコンポーネントの情報を参照してください

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ermenegilda Vitale et al.
Plants (Basel, Switzerland), 10(5) (2021-05-01)
This paper evaluates the combined effect of biostimulant and light quality on bioactive compound production and seedling growth of soybean (Glycine max L. Merrill) plants. Germinated seeds pre-treated with different concentrations (0.01%, 0.05%, 0.5%) of an amino acid-based biostimulant were
Giulia Costanzo et al.
Antioxidants (Basel, Switzerland), 9(6) (2020-06-18)
Peel, pulp and seed extracts of three mandarin varieties, namely Phlegraean mandarin (Citrus reticulata), Kumquat (Citrus japonica), and Clementine (Citrus clementina) were compared and characterised in terms of photosynthetic pigment content, total polyphenols amount, antioxidant activity and vitamin C to
Giulia Costanzo et al.
Antioxidants (Basel, Switzerland), 11(2) (2022-02-26)
In this work, we assess the potential of waste products of Phlegrean mandarin (Citrus reticulata Blanco), namely seeds and peel, to be reutilized as a source of bioactive compounds beneficial for the human diet. Starting from the evidence that the
Manas R Gartia et al.
Scientific reports, 5, 16011-16011 (2015-11-04)
Limited training, high cost, and low equipment mobility leads to inaccuracies in decision making and is concerning with serious ocular injuries such as suspected ruptured globe or post-operative infections. Here, we present a novel point-of-service (POS) quantitative ascorbic acid (AA)
Stephanie P DiTroia et al.
Nature, 573(7773), 271-275 (2019-09-06)
Development is often assumed to be hardwired in the genome, but several lines of evidence indicate that it is susceptible to environmental modulation with potential long-term consequences, including in mammals1,2. The embryonic germline is of particular interest because of the
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service