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M8413

Sigma-Aldrich

Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody produced in mouse

clone 35509.11, purified immunoglobulin, lyophilized powder

Synonym(s):

Anti-MCP-2

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About This Item

MDL number:
MFCD01092422
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

35509.11, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

indirect ELISA: suitable
neutralization: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

P80075

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CCL8(6355)

General description

Monocyte Chemotactic Protein-2 (MCP-2) belongs to a family of C-C chemotactic cytokines. MCP-1, -3, -4, and -5 are the other five monocyte chemo attractants identified. The MCP cytokines are involved in recruitment of leukocytes to the sites of inflammation, and activate NK cells and CD4+ and CD8+ T lymphocytes. Additionally, MCP cytokines can activate basophils to secrete histamines and also attract eosinophils. MCPs play an important role in pathological conditions such as systemic sclerosis, myocardia and cardiac ischemia. Anti-monocyte chemotactic protein 2 antibody specifically reacts with human MCP-2. The antibody shows <20% cross reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.

Specificity

Neutralizes the biological activity of recombinant human MCP-2. Shows <20% cross-reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.

Immunogen

recombinant human MCP-2 expressed in E. coli.

Application

Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody may be used in ELISA at a concentration of 2.0 μg/ml. For immunoblotting a working concentration of 1-2 μg/ml may be used. The antibody is suitable for neutralization reactions; ND50 is 1-4 μg/ml.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose

Preparation Note

Purified using protein A.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

M8413-.5MG-PW:
M8413-BULK:
M8413-.5MG:
M8413-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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I Clark-Lewis et al.
Journal of leukocyte biology, 57(5), 703-711 (1995-05-01)
Structural analysis of chemokines has revealed that the alpha/beta structural-fold is highly conserved among both the CXC and CC chemokine classes. Although dimerization and aggregation is often observed, the chemokines function as monomers. The critical receptor binding regions are in
Jianli Niu et al.
Clinical science (London, England : 1979), 117(3), 95-109 (2009-07-02)
Many of the major diseases, including cardiovascular disease, are widely recognized as inflammatory diseases. MCP-1 (monocyte chemotactic protein-1) plays a critical role in the development of cardiovascular diseases. MCP-1, by its chemotactic activity, causes diapedesis of monocytes from the lumen
J H W Distler et al.
Rheumatology (Oxford, England), 48(2), 98-103 (2008-11-06)
Activation of the immune system and increased synthesis of extracellular matrix proteins by fibroblasts are hallmarks in the pathogenesis of SSc. The molecular mechanisms underlying the infiltration of inflammatory cells into the skin and the subsequent activation of fibroblasts are
M Weber et al.
Journal of immunology (Baltimore, Md. : 1950), 154(8), 4166-4172 (1995-04-15)
It has been shown that CC chemokines activate basophil and eosinophil leukocytes with different selectivities and patterns of activity. The most effective are monocyte chemotactic protein-1 (MCP-1), a potent stimulus of mediator release in basophils without effects on eosinophils, RANTES

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