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M5904

Sigma-Aldrich

Mineral oil

for molecular biology, BioReagent, light oil

Synonym(s):

light mineral oil

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About This Item

CAS Number:
8042-47-5
EC Number:
232-455-8
MDL number:
MFCD00131611
UNSPSC Code:
12181504
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

product line

BioReagent

form

light oil

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

suitability

suitable for overlaying aqueous reactions and centrifuge gradients

foreign activity

DNase, RNase, Protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oils are usually seen as a mixture of liquid hydrocarbons. It is derived from crude oil by distillation and refining.

Application

Mineral oil has been used:
  • to suspend the gels for capillary contraction assay
  • in polymerase chain reaction (PCR) reactions mixture to form a vapor barrier in order to prevent the evaporation of the reaction at elevated temperatures
  • as a continuous phase to prevent droplet coalescence in emulsification system

Mineral oil is suitable for use as an overlay to control evaporation and cross contamination of samples in a variety of molecular biology applications.

Biochem/physiol Actions

Mineral oil delivers a better insulation strength, when compared to air. It enhances the elimination of heat from the windings, when used in a power transformer.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Group 4: Flammable liquids
Type 3 petroleums
Hazardous rank III
Water insoluble liquid

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

M5904-5X5ML:4548173203034
M5904-VAR:
M5904-BULK:
M5904-5ML:4548173203027
M5904-6X500ML:4548173203041
M5904-500ML:4548173203010

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A quantitative analysis of contractility in active cytoskeletal protein networks
Bendix PM, et al.
Biophysical Journal, 94(8), 3126-3136 (2008)
Microfluidic flow-focusing in ac electric fields
Tan SH, et al.
Lab on a chip, 14(6), 1099-1106 (2014)
Process Fluids for Abrasive Machining
Tribology of Abrasive Machining Processes, 531-585 (2004)
S Nathakarnkitkool et al.
Electrophoresis, 13(1-2), 18-31 (1992-01-01)
Capillary electrophoretic separations of double-stranded DNA fragments in the size range of 20-2200 base pairs were achieved in less than 20 min with the use of a Tris-borate buffer containing hydroxyethylcellulose. Analyses were carried out in both uncoated and phenylmethyl-coated
Construction of a full-length enriched and a 5'-end enriched cDNA library using the oligo-capping method.
Yutaka Suzuki et al.
Methods in molecular biology (Clifton, N.J.), 221, 73-91 (2003-04-22)
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