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M5634

Sigma-Aldrich

5-Mercuricytidine carbonate 5′-triphosphate triethylammonium salt

~90%

Synonym(s):

Hg-CTP

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About This Item

Linear Formula:
C9H15HgN3O14P3 · HCO3
CAS Number:
100940-62-3
Molecular Weight:
1148.52
MDL number:
MFCD00058366
UNSPSC Code:
41106305
PubChem Substance ID:
329818019
NACRES:
NA.51

Assay

~90%

form

solid

storage temp.

−20°C

SMILES string

CCN(CC)CC.NC1=NC(=O)N(C=C1[Hg])[C@@H]2O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]2O

InChI

1S/C9H15N3O14P3.C6H15N.Hg/c10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(24-8)3-23-28(19,20)26-29(21,22)25-27(16,17)18;1-4-7(5-2)6-3;/h2,4,6-8,13-14H,3H2,(H,19,20)(H,21,22)(H2,10,11,15)(H2,16,17,18);4-6H2,1-3H3;/t4-,6-,7-,8-;;/m1../s1

InChI key

CKMVNPSLWQKVPG-WFIJOQBCSA-N

Application

5-Mercuricytidine carbonate 5;-triphosphate (Hg-CTP) may be used as a substrate to generate mercurated DNA that may be suitable for image based DNA sequencing strategies.

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

Signal Word

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - STOT RE 2

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Poisonous substance

PRTR

Class I Designated Chemical Substances

JAN Code

M5634-BULK:
M5634-5MG:4548173202969
M5634-10MG:4548173279718
M5634-1MG:4548173279725
M5634-VAR:

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Study of deoxyribonucleic acid replication in permeable cells of Bacillus subtilis using mercurated nucleotide substrates.
S Bhattacharya et al.
Biochemistry, 20(11), 3029-3034 (1981-05-26)
G Banfalvi et al.
Analytical biochemistry, 146(1), 64-70 (1985-04-01)
A method for isolating picomole quantities of nascent mercurated DNA from a mixture of cellular nucleic acids using affinity chromatography on thiol-agarose is described. Analysis of mercurated DNA (HgDNA) isolated in the presence of in vivo-labeled cellular RNA or in
R M Dale et al.
Proceedings of the National Academy of Sciences of the United States of America, 70(8), 2238-2242 (1973-08-01)
A simple acetoxymercuration reaction for introducing covalently bound mercury atoms into nucleotides is described. The 5-mercuriacetate derivatives of UTP, CTP, dUTP, and dCTP, as well as the 7-mercuriacetate derivative of 7-deazaATP, have been prepared by this procedure and tested as
S Bhattacharya et al.
The Journal of biological chemistry, 257(4), 1610-1612 (1982-02-25)
Mercurated DNA was synthesized in permeable cells of Bacillus subtilis, using 5-mercurideoxycytidine triphosphate as one of the substrates, and was separated from parental unsubstituted DNA by isopycnic centrifugation in CsCl gradients. The ability of mercurated DNA to transform auxotrophic strains
A J Bridgman et al.
DNA sequence : the journal of DNA sequencing and mapping, 6(4), 199-209 (1996-01-01)
The development of high-resolution scanning-probe microscopes has reawakened interest in the possibility of sequencing large nucleic acid molecules by direct imaging. Such an approach would be facilitated by the availability of effective methods for increasing contrast by labelling specific nucleotides
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