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M0268

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used:
  • in the isolation and culture of primary hepatocytes
  • to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
  • to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

Features and Benefits

  • Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
  • The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Essentially the same as the original formula, except it is provided as a powder.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

M0268-2X5L:
M0268-50L:
M0268-10X1L:
M0268-25KG:
M0268LF1-BIBC:
M0268-10L:
M0268-PROC:
M0268-BIBC:
M0268-VAR:
M0268-100L:
M0268-50K:
M0268-25K:
M0268-50KG:
M0268-1L:
M0268-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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