L9152

Anti-Leukemia Inhibitory Factor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

• Synonym(s): CDF, D factor, DIA, Emfilermin, LIF, differentiation inhibitory activity
• MDL number: MFCD00283185
• UNSPSC Code: 51111800
• NACRES: NA.43

Properties

• biological source: goat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: mouse
• technique(s): immunohistochemistry: suitable; neutralization: suitable; western blot: suitable
• UniProt accession no.: P09056
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LIF(3976) ; mouse ... Lif(16878)

Description

Specificity

The antibody neutralizes the biological activity of recombinant mouse LIF and also neutralizes the biologicl activity of recombinant human LIF at a 10-fold higher IgG concentration. Based on ELISA and immunoblotting, this antibody shows <10% cross-reactiity with recombinant human LIF.

Immunogen

purified, E. coli-derived, recombinant mouse LIF.

Application

For Western blot a concentration of 0.1 μg/ml is recommended. For immunohistochemistry, a working dilution of 5-15 μg/ml is recommended. The neutralization dose is typically 80-480 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.

Optimal dilutions should be determined by the end user.

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Preparation Note

Protein A or G purified

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves