Refer to the product information sheet (under Documents, above) for L4031 for preparation information.
L4395
Liposome Kit: Lipid mixtures for the preparation of liposomes
lyophilized powder
Synonym(s):
cholesterol, L-α-phosphatidylcholine and stearylamine kit
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1 VIAL
¥25,800
5 VIALS
¥87,100
About This Item
Recommended Products
biological source
egg yolk
Quality Level
form
lyophilized powder
composition
Cholesterol, 9 μmol/package
L-α-Phosphatidylcholine (egg yolk), 63 μmol/package
Stearylamine, 18 μmol/package
functional group
carboxylic acid
shipped in
ambient
storage temp.
−20°C
Related Categories
Biochem/physiol Actions
Liposome Kit: Lipid mixtures for the preparation of liposomes contains cholesterol; L-α-phosphatidylcholine and stearylamine. Liposome kit may be used to prepare cationic (positively charge) liposomes for incorporation of materials into cells.
Liposomes can be used for different applications such as drug delivery, diagnostics, membrane models, adjuvants for vaccination and others. This lipid mixture can be used to encapsulate a broad spectrum of hydrophilic and amphipathic molecules of low, medium, and high molecular weight (including peptides, proteins, and oligo- and polynucleotides).
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
PRTR
Class I Designated Chemical Substances
JAN Code
L4395-BULK:
L4395PROC:
L4395-1VL-PW:
L4395-PH:
L4395-VAR:
L4395-5VL:
L4395-1VL:
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Rui Xu et al.
Analytical chemistry, 88(21), 10390-10394 (2016-11-02)
Chemical analysis of small extracellular vesicles (sEVs) circulating in body fluids holds potentials in noninvasive diagnosis of diseases and evaluation of therapeutic treatments. However, quantification of sEVs remains a challenge due to lacking of cost-effective analytical protocols. Herein we report
Quantification of small extracellular vesicles by size exclusion chromatography with fluorescence detection
Xu R, et al.
Analytical Chemistry, 88(21), 10390-10394 (2016)
Abstracts of the Congress of the European Association of Nuclear Medicine. 25-29 August 2001, Napoli, Italy.
European journal of nuclear medicine, 28(8), 961-1298 (2001-09-06)
S Palakurthi et al.
International journal of pharmaceutics, 290(1-2), 55-62 (2005-01-25)
Conventional lipid microspheres (LM) were prepared using soybean oil and lipid at a 5.5:1 weight ratio with lipid phase consisting of PC (phosphatidyl choline):CH (cholesterol) (1:0.5) by molar ratio. The average diameter of the particles was 150 nm. Long-circulating microspheres
Evaluation of intracellular and extracellular trehalose as a cryoprotectant of stem cells obtained from umbilical cord blood
Motta JPR, et al.
Cryobiology, 68(3), 343-348 (2014)
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How do I prepare the Product L4395, Liposome Kit liposomes?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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I have purchased Product L4395, Liposome Kit. Is there a difference between positive and negative liposomes?
1 answer-
The choice is dependent upon what is being encapsulated and how it is being used. A good reference discussing this question is Ogiso, T., et al., Effect of positively and negatively charged liposomes on skin permeation of drugs. J. Drug Target, 9(1), 49-59 (2001).
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I'm thinking of purchasing Product L4395, Liposome Kit. Why would one use positive vs. negative liposomes?
1 answer-
Analogous to poly-L-lysine, one may need positively charged agents to deliver drugs to cells, hence positive liposomes.
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How is Product L4395, Liposome Kit, used?
1 answer-
The Liposomes of a large multilamellar structure type are the most widely used. They can be prepared using Sigma liposome mixtures by concentrating an organic solvent solution of lipids to a thin film on the walls of a container such as by rotary evaporation or by drying in glass with a stream of nitrogen gas. Then, under appropriate conditions, an aqueous solution is added to the thin film and with agitation multilamellar vesicles should form spontaneously with little effort. Large or small unilamellar structured liposomes can also be prepared by varying procedure or conditions. Please see the product insert for Product No. L4031 (the link below) for a step by step protocol that will work for this product also.
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