Skip to Content
Merck
All Photos(1)

Documents

Safety Information

L3289

Sigma-Aldrich

Lysis solution for blood

sufficient for 100 reactions, for molecular biology

Synonym(s):

Blood direct PCR, Blood lysis buffer, Extract-N-Amp blood direct PCR lysis solution

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

grade

for molecular biology

form

liquid

usage

sufficient for 100 reactions

storage temp.

−20°C

Related Categories

General description

Lysis solution for blood in combination with neutralization solution for blood (N9784) aids in rapid extraction and neutralization of DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

This lysis solution is a component of the Extract-N-Amp™ and the REDExtract-N-Amp™ Blood PCR Kits

Application

Lysis solution for blood has been used for lysis of blood cells for DNA extraction prior to polymerase chain reaction (PCR) amplification. It has also been used for the isolation of genomic DNA for CRISPR-Cas9 gene editing.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

XNAB

REDExtract-N-Amp™ Blood PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNABR

REDExtract-N-Amp™ Blood PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAB2

Extract-N-Amp™ Blood PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNABE

REDExtract-N-Amp™ Blood PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications

XNAB2RE

Extract-N-Amp™ Blood PCR Kit, sufficient for 1000 extractions, sufficient for 5000 amplifications

XNABS

REDExtract-N-Amp™ Blood PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

P8240

REDExtract-N-Amp™ PCR ReadyMix™ for Blood, 12 mL sufficient for 1000 amplifications

P8115

Extract-N-Amp™ PCR ReadyMix™ for Blood, 12 mL sufficient for 1000 amplifications

N9784

Neutralization Solution for Blood, sufficient for 100 reactions, sufficient for 1000 reactions, for molecular biology

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H290,H314

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

L3289-25ML:
L3289-.3ML:
L3289-2.5ML:4548173925394
L3289-.3ML-KC:
L3289-BULK:
L3289PROC:
L3289-VAR:
L3289-25ML-KC:
L3289-2.5ML-KC:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and Cellular Biology, MCB-00790 (2015)
Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and cellular biology, MCB-00790 (2015)
Shuhan Chen et al.
Stem cell research, 45, 101804-101804 (2020-04-28)
Mutations in the Leucine rich repeat kinase 2 (LRRK2) gene are found in both familial and sporadic Parkinson's disease (PD), and are also associated with immune-related disorders including Crohn's disease (CD) and leprosy. We have generated two homozygous LRRK2 knockout
Meng Zhang et al.
Stem cell research, 41, 101602-101602 (2019-11-08)
Loss of function mutations in PARK2 (encoding PARKIN) cause autosomal recessive Parkinson's disease (PD), which often manifests at a juvenile age. Molecular and biochemical studies show that PARKIN functions as an E3 ubiquitin ligase controlling mitochondrial homeostasis. Yet, the exact
Huiling Wu et al.
The Journal of clinical investigation, 117(10), 2847-2859 (2007-09-15)
Ischemia/reperfusion injury (IRI) may activate innate immunity through the engagement of TLRs by endogenous ligands. TLR4 expressed within the kidney is a potential mediator of innate activation and inflammation. Using a mouse model of kidney IRI, we demonstrated a significant
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service