L2895

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B₄ (BSI-B₄), FITC conjugate, lyophilized powder

• Synonym(s): Bandeirea simplicifolia agglutinin, BS-I
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• conjugate: FITC conjugate
• Quality Level: 200
• form: lyophilized powder
• potency: <50 μg per mL agglutination activity (using human blood group B erythrocytes)
• composition: Protein, ≥70% Lowry (balance sodium citrate)
• extent of labeling: ≥1 mol FITC per mol lectin
• storage temp.: 2-8°C

Description

General description

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.[1]

Application

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for microglia detection[2] in murine astrocytes culture by staining the culture with lectin BS1-B4.[3] It has also been used in the preparation of staining solution to incubate the tissue sections.[4]

Biochem/physiol Actions

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling, because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.[1]

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Packaging

Package size based on protein content by Lowry.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B₄, BSI-AB₃, BSI-A₂B₂, BSI-A₃B and BSI-A₄. BSI-B₄ is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A₄ has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves