KEM0033

T4 DNA Polymerase

Ultra-pure enzyme for nucleic acid modifications

Properties

• grade: for molecular biology
• Assay: >99% (SDS-PAGE)
• form: buffered aqueous solution
• specific activity: 5,555 U/mg
• concentration: 3,000 U/mL
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′ → 3′ direction. This enzyme exhibits a powerful 3′ → 5′ exonuclease activity, while lacking any inherent 5′ → 3′ exonuclease or strand displacement functions.

Application

Suitable for DNA end-repair by fill-in of 5′ overhang and removal of 3′ overhang.

Features and Benefits

• Ultra-purification process for ultimate enzyme performance
• Highest quality specifications for ultimate product consistency
• Undetectable DNA and nuclease contamination

Components

Supplied with:KEM0042B (10X Blue Buffer)

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37° C.

Physical form

Supplied in 100 mM KPO₄, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 6.5 @ 25° C.

Other Notes

Source of protein: Purified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.

Unit size: 2,000 U

Safety Information

• Storage Class Code: 10 - Combustible liquids