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I5654

Sigma-Aldrich

IgG3, Kappa from human myeloma plasma

purified immunoglobulin, >90% (microfluidic capillary gel electrophoresis)

Synonym(s):

Human IgG3-κ

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About This Item

MDL number:
MFCD00163926
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

human

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

Assay

>90% (microfluidic capillary gel electrophoresis)

shipped in

dry ice

storage temp.

−20°C

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General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG3 is the has a unique extended hinge region conferring greater flexibility
Human myeloma IgG3, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.

Application

IgG3, kappa from human myeloma plasma has been used to determine the trisulfide levels in commercial therapeutic IgG1 antibodies and in human myeloma IgG1, IgG2, IgG3 and IgG4 antibodies.
The purified IgG3, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG3 was for in flow cytometry in CHO cells.

Physical form

Solution in 20 mM tris buffered saline, pH 8.0.

Analysis Note

The purity and identity are determined by immunoelectrophoresis, indirect ELISA and SDS-PAGE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

I5654PROC:
I5654-1MG-PW:
I5654-1MG:
I5654-VAR:
I5654-PH:
I5654-BULK-N:
I5654-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Characterization of trisulfide modification in antibodies
Gu S, et al.
Analytical Biochemistry, 400(1), 89-98 (2010)
Lela Kardava et al.
Nature immunology, 19(9), 1001-1012 (2018-08-15)
Immunoglobulin G3 (IgG3) has an uncertain role in the response to infection with and vaccination against human immunodeficiency virus (HIV). Here we describe a regulatory role for IgG3 in dampening the immune system-activating effects of chronic HIV viremia on B
Hong-Sheng Dai et al.
Immunity, 47(1), 159-170 (2017-07-21)
Clearance of pathogens or tumor cells by antibodies traditionally requires both Fab and Fc domains of IgG. Here, we show the Fc domain of IgG alone mediates recognition and clearance of herpes simplex virus (HSV1)-infected cells. The human natural killer
Rangaiah Shashidharamurthy et al.
Journal of immunology (Baltimore, Md. : 1950), 183(12), 8216-8224 (2009-12-17)
CD32A, the major phagocytic FcgammaR in humans, exhibits a polymorphism in the ligand binding domain. Individuals homozygous for the R allelic form of CD32A (CD32A(R) allele) are more susceptible to bacterial infections and autoimmune diseases as compared with H allelic
Masayuki Hirano et al.
Nature immunology, 8(7), 762-771 (2007-06-15)
Because functional analysis of Fc receptors (FcRs) relies heavily on mouse models, the identification of another Fcgamma receptor is particularly noteworthy. We demonstrate that FcgammaRIV, identified here as the mouse ortholog of primate FcgammaRIII, required association of the FcR gamma-chain
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