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H9250

Sigma-Aldrich

Histone from calf thymus

Type II-A, lyophilized powder

Synonym(s):

histone type VIII-S

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About This Item

CAS Number:
9064-47-5
Beilstein:
1895465
MDL number:
MFCD00131329
UNSPSC Code:
41106305
NACRES:
NA.51

biological source

bovine (calf) thymus

type

Type II-A

form

lyophilized powder

application(s)

cell analysis

storage temp.

2-8°C

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General description

Histones are octameric proteins containing H3, H4, H2A and H2B. It is the core protein around which DNA is wound forming the nucleosome structure. The core histone proteins contain globular domains. Each protein is composed of basic amino acids at its N-terminal. The H2A has an extra amino acid at its C-terminal end forming a histone tail. The nucleosome structure is stabilized by the linker histones during chromatin condensation.

Application

Histone from calf thymus has been used:
  • in methyltransferase reaction mixture for methyltransferase assay
  • in in vitro binding assay, to explore the potential binding of the MORF4 (mortality factor on chromosome 4) - related gene on chromosome 15 (MRG15) chromo domain with histone
  • in in vitro coculture assays, to stimulate liver resident Kupffer cells

Biochem/physiol Actions

The core histones are involved in histone-histone interactions and maintains the organization of the nucleosomal DNA. The histone tail helps in forming higher-order nucleosomal structures. The overall structure and action of chromatin fiber is regulated by histone modifications.

Features and Benefits

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Preparation Note

Unfractionated whole histone

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

H9250-VAR:
H9250-500MG:
H9250-100MG:
H9250-BULK:
H9250-1G:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Histones activate the NLRP3 inflammasome in Kupffer cells during sterile inflammatory liver injury
Huang H, et al.
Journal of Immunology, 191(5), 2665-2679 (2013)
Juan-Carlos Cabrera et al.
Glycobiology, 20(6), 775-786 (2010-03-05)
Plant cell walls undergo remodeling during growth and development and are the first target of many invading pathogens. Acidic pectin (homogalacturonans) binds calcium and forms chain dimers called egg boxes and even multimers at higher calcium ion concentrations. Chitosan, the
Histones and histone modifications
Peterson CL, et al.
Current Biology, 14(14), R546-R551 (2004)
Yannick Tauran et al.
Scientific reports, 9(1), 5816-5816 (2019-04-11)
By means of Silicon Nano Tweezers (SNTs) the effects on the mechanical properties of λ-phage DNA during interaction with calf thymus nucleosome to form an artificial chromatin analog were measured. At a concentration of 100 nM, a nucleosome solution induced a
Armin M Gamper et al.
Molecular and cellular biology, 29(1), 266-280 (2008-10-22)
Human STAGA is a multisubunit transcriptional coactivator containing the histone acetyltransferase GCN5L. Previous studies of the related yeast SAGA complex have shown that the yeast Gcn5, Ada2, and Ada3 components form a heterotrimer that is important for the enzymatic function
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