GL0010

Golgi Isolation Kit

sufficient for 50 g (tissue)

• Synonym(s): Golgi Kit, Isolation Kit for Golgi
• NACRES: NA.32
• UNSPSC Code: 12352200

Properties

• Quality Segment: 200
• usage: sufficient for 50 g (tissue)
• technique(s): fractionation: suitable
• input: mammalian sample(s)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).

Application

Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.

Analysis Note

The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.

Kit Components Also Available Separately

• P8340

  Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL

  SDS

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3