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GERPN2236

ECL™ Prime Western Blotting Detection Reagent

Cytiva RPN2236

Synonym(s):

chemiluminescent detection reagent, Western blotting detection reagent

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About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN2236

General description

Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.

Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.

Application

ECL™ Prime Western blotting detection reagent has been used to detect chemiluminescent signals during Western blotting.

Features and Benefits

  • Compatible with Rainbow Molecular Weight Markers and Amersham™ ECL DualVue™ Western Blotting Markers.
  • High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
  • Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
  • Optimized for use with Amersham Hybond-P® (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.
  • Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm™.

Components

The following components are included in the Amersham ECL Prime detection reagent kit.
  • RPN2236 Solution A: Luminol solution, 3 × 50 ml Solution B: Peroxide solution, 3 × 50 ml sufficient for 3000 cm2 membrane

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
ECL DualView is a trademark of Amersham
Hybond is a registered trademark of Cytiva
Hyperfilm is a trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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eLife, 3 (2014)

Articles

Methods Used to Detect Proteins and Nucleic Acids Bound to Membranes

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

Troubleshooting Detection Methods for GST-tagged Proteins

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

Protocols

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service