GE17-5156-01
HiTrap® Q Fast Flow
Cytiva 17-5156-01, pack of 5 × 5 mL
Synonym(s):
HiTrap Q Column
About This Item
Recommended Products
ligand
quaternary amine
description
Ion Exchanger Type (value)
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5156-01
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
2-12
suitability
suitable for bioprocess medium
Related Categories
General description
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
Features and Benefits
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High flow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
Storage and Stability
Analysis Note
Other Notes
Legal Information
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
Protocols
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Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page covers the use of Sepharose Fast Flow for purification of proteins.
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