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G1924

Sigma-Aldrich

L-Glutamate Oxidase from Streptomyces sp.

recombinant, expressed in E. coli

Synonym(s):

L-Glutamate:oxygen oxidoreductase (deaminating)

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About This Item

CAS Number:
39346-34-4
MDL number:
MFCD00131459
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

form

solid

specific activity

≥5 units/mg protein

mol wt

120 kDa

storage temp.

−20°C

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General description

L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.
This product has a 6xHistidine tag with a thrombin cleavage site.

Application

L-Glutamate Oxidase from Streptomyces sp may be used:
  • as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
  • as a component of pencil graphite electrode for biosensing L-glutamate

Biochem/physiol Actions

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.

Unit Definition

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

G1924-BULK:
G1924-5UN-PW:
G1924-VAR:
G1924-5UN:
G1924-1UN-PW:
G1924-PH:
G1924PROC:
G1924-1UN:
I-3622:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Comparative study of hydrogel-immobilized L-glutamate oxidases for a novel thick-film biosensor and its application in food samples
Kwong AWK, et al.
Biotechnology Letters, 22(4), 267-272 (2000)
L-Glutamate biosensor based on l-glutamate oxidase immobilized onto ZnO nanorods/polypyrrole modified pencil graphite electrode
Batra B, et al.
Biochemical Engineering Journal, 105, 428-436 (2016)
Isolation, purification, and characterization of L-glutamate oxidase from Streptomyces sp. 18G
Wachiratianchai S, et al.
Electronic journal of Biotechnology, 7(3), 09-10 (2004)
Jiro Arima et al.
The FEBS journal, 276(14), 3894-3903 (2009-06-18)
L-Glutamate oxidase (LGOX) from Streptomyces sp. X-119-6, which catalyzes the oxidative deamination of L-glutamate, has attracted increasing attention as a component of amperometric L-glutamate sensors used in the food industry and clinical biochemistry. The precursor of LGOX, which has a
Andrea C Kneuttinger et al.
Cell chemical biology, 26(11), 1501-1514 (2019-09-10)
Imidazole glycerol phosphate synthase (ImGPS) is an allosteric bienzyme complex in which substrate binding to the synthase subunit HisF stimulates the glutaminase subunit HisH. To control this stimulation with light, we have incorporated the photo-responsive unnatural amino acids phenylalanine-4'-azobenzene (AzoF)

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