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F1879

Sigma-Aldrich

Formaldehyde Dehydrogenase from Pseudomonas sp.

lyophilized powder, 1.0-6.0 units/mg solid

Synonym(s):

FDH, Formaldehyde:NAD+ oxidoreductase

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About This Item

CAS Number:
9028-84-6
Enzyme Commission number:
1.2.1.46 (BRENDA, IUBMB)
MDL number:
MFCD00131100
UNSPSC Code:
12352204

form

lyophilized powder

specific activity

1.0-6.0 units/mg solid

storage temp.

−20°C

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General description

Formaldehyde Dehydrogenase (FDH) belongs to the medium-chain alcohol dehydrogenase family. It is encoded by the fdhA and fdhB gene in Pseudomonas aeruginosa. The FDH sequences of P. aeruginosa and P. putida are conserved and show homology. FDH exists as dimer and tetramer with zinc in their catalytic active site. The monomer corresponds to 350-400 amino-acid residues.

Application

Formaldehyde Dehydrogenase from Pseudomonas sp. has been used:

  • For standard curve generation for the demethylation-FDH assay of lysine specific demethylase 1 (LSD1) and in formaldehyde dehydrogenase (FDH) assay.
  • As a biosensor for the presence of formaldehyde in pharmaceuticals, waste water, vaccines and industrial products.
  • In coupled pectin methyl esterase (PME) enzyme assay.

Biochem/physiol Actions

Formaldehyde dehydrogenase (FDH) is an enzyme that catalyzes the oxidation of formaldehyde to formate with NAD+ as an electron acceptor.Formaldehyde dehydrogenases are involved in the biological detoxification of formaldehyde, a toxic product in the metabolism of compounds like histidine, vanillate etc. They detoxify by converting formaldehyde to carbon-di-oxide. FDH plays a crucial role in regulating protein S-nitrosation in mammalian cells by decomposing S-nitrosoglutathione. They are also involved in the regulation of nitrosothiol homeostasis by catalyzing the reduction of endogenous nitrosylating agent S-nitrosoglutathione (GSNO).

Unit Definition

One unit will oxidize 1.0 μmole of formaldehyde to formic acid per min at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing ~70% stabilizers as Mg2+, Ca2+, bovine serum albumin, glycine, and lysine

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

F1879-5UN:
F1879-25UN:
F1879-VAR:
F1879-50UN:
F1879-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The role of a formaldehyde dehydrogenase-glutathione pathway in protein S-nitrosation in mammalian cells
Haqqani A S, et al.
Nitric Oxide, 9(3), 172-181 (2003)
Structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa: the binary complex with the cofactor NAD+
Liao Y, et al.
Acta Crystallographica. Section F, Structural Biology Communications, 69(9), 967-972 (2013)
Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa
Zhang W, et al.
Protein Expression and Purification, 92(2), 208-213 (2013)
In Vitro Enzyme Assays for JmjC-Domain-Containing Lysine Histone Demethylases (JmjC-KDMs)
Tarhonskaya H, et al.
Current protocols in pharmacology, 80(1), 3-15 (2018)
Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa
Zhang W, et al.
Protein Expression and Purification, 92(2), 208-213 (2013)
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