DMN10

GenElute^™ Direct mRNA Miniprep Kits

sufficient for 10 purifications

• Synonym(s): GenElute^™ Direct mRNA Miniprep Kit, GenElute^™ mRNA Kit, Gen Elute
• UNSPSC Code: 41105501
• NACRES: NA.52

Properties

• usage: sufficient for 10 purifications
• technique(s): RNA purification: suitable
• storage temp.: 15-25°C

Description

General description

The GenElute^™ Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 10⁷ mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Application

The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Features and Benefits

• mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
• Poly (A)⁺ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
• Oligo(dT) polystyrene beads require fewer wash steps

Other Notes

Use for isolating mRNA directly from mammalian cells or tissues.

For additional information, please see www.sigma-aldrich.com/mrna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Elution solution 1.5 mL
• Filtration columns with tubes 10 ea
• Lysis solution 20 mL
• 5 M NaCl 1.5 mL
• Oligo(dT)-polystyrene beads .3 mL
• Proteinase K 5 mg
• 40% Glycerol solution .6 mL
Spin columns with tubes 10 ea
Collection tube 10 ea

See All (9)

Safety Information

• Pictograms: GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H290,H315,H319,H334,H335
• Precautionary Statements: P234 - P261 - P264 - P271 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable