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D5652

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

Synonym(s):

DPBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.25

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

pH

7.2-7.8

solubility

water: 9.6 g/L, clear

storage temp.

2-8°C

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General description

Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.

Application

D-PBS is used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
  • as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy
  • to dissolve Angelica gigas powder for microencapsulation
  • to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay
  • to wash amniotic membrane for cellular analyses

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Other Notes

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D5652LF1-BIBC:
D5652-2000L:
D5652-5L:
D5652-BULK:
D5652-VAR:
D5652-25K:
D5652-PROC:
D5652-25KG:
D5652-2X5L:
D5652-50KG:
D5652-BIBC:
D5652-200L:
D5652-1L:
D5652-10X1L:
D5652-50L:
D5652-50K:
D5652-10L:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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