Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

D4764

Sigma-Aldrich

Deoxyribonucleic acid from calf thymus

Genomic, unsheared

Synonym(s):

double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
91080-16-9
EC Number:
293-507-3
MDL number:
MFCD00151698
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

form

lyophilized powder

mol wt

>20 kb

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Specificity

42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D4764-VAR:
D4764-1UN:
D4764PROC:
D4764-BULK:
D4764-.25UN:
D4764-5UN:

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000353056
0000322783
0000322784
0000295449
0000295448

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Roel J H Custers et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 28(4), 429-435 (2009-11-04)
The purposes of the present study were to explore the surgical possibilities for replacement of the medial tibial plateau by a metallic implant in a large animal model and to examine the implications for the opposing cartilage. In six goats
A Châtel et al.
Ecotoxicology (London, England), 20(8), 1727-1740 (2011-06-10)
In the present study, we analyzed the effects of two major pollutants of the environment, tributyltin (TBT) and water-accommodated fraction (WAF) of diesel oil, on MAP kinase activation, apoptosis induction and DNA damage, in the marine sponge Suberites domuncula. Our
Nathalie W D Jansen et al.
Arthritis and rheumatism, 56(1), 199-207 (2006-12-30)
Joint bleeding, or hemarthrosis, leads in time to severe joint damage. This study was carried out to test the in vitro thresholds of exposure time and concentration that lead to irreversible joint damage, to add to the discussion on the
José Luis García-Giménez et al.
Journal of inorganic biochemistry, 121, 167-178 (2013-02-07)
Ternary copper(II) complexes [Cu(NST)2(phen)] (1) and [Cu(NST)2(NH3)2]·H2O (2) [HNST=N-(4,5-dimethylthiazol-2-yl)naphthalene-1-sulfonamide] were prepared and characterized by physico-chemical techniques. Both 1 and 2 were structurally characterized by X-ray crystallography. The crystal structures show the presence of a distorted square planar CuN4 geometry in
K Bishayee et al.
Cell proliferation, 46(2), 153-163 (2013-03-21)
Small aromatic compounds like flavonoids can intercalate with DNA molecules bringing about conformational changes leading to reduced replication and transcription. Here, we have examined one dietary flavonoid, quercetin (found in many fruit and vegetables), for possible anti-cancer effects, on HeLa
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service