D3571

Dipeptidyl Peptidase III human

recombinant, expressed in Sf9 cells

• Synonym(s): DPP III
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥400 units/μg protein
• Recombinant: expressed in Sf9 cells
• Concentration: ≥0.1 mg/mL

Properties

• recombinant: expressed in Sf9 cells
• Quality Segment: 200
• form: solution
• specific activity: ≥400 units/μg protein
• mol wt: 82 kDa
• concentration: ≥0.1 mg/mL
• NCBI accession no.: NM_005700, NP_005691
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... DPP3(10072)

Description

Application

Human dipeptidyl peptidase III has been used in a study to assess the effect of entropy-driven binding of opioid peptides on large domain motion in human dipeptidyl peptidase III. Human dipeptidyl peptidase III has also been used in a study to investigate Ets-1/Elk-1 as a critical mediator of its transcription in human glioblastoma cells.

Biochem/physiol Actions

DPP III is a cytosolic zinc-exopeptidase that is involved in the intracellular protein catabolism of eukaryotes. The enzyme is a monomeric acidic protein with a molecular mass of approximately 82,000 Da and a pI of 4.5-4.6. It is sensitive to freezing and temperatures above 40 °C. It is found to be inhibited by metallo-chelators and sulfydryl reagents. The activity can be restored by divalent cations and thiol compounds. It has a particularly high affinity for angiotensin III.[1] It acts as a post-proline-cleaving enzyme on endomorphins.[2]

Physical form

Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol and 3 mM DTT.

Other Notes

One unit will hydrolyze 1.0 picomole of Arg-Arg-AMC per minute at pH 7.5 at 25 deg °C

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable