CMC0003

SIG10 HIGH Electrocompetent Cells

for protein expression and DNA plasmid production

• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• biological source: Escherichia coli
• grade: for molecular biology
• form: buffered aqueous solution
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: electrocompetent; transformation efficiency: ≥5 × 10⁹ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The SIG10 HIGH Electrocompetent Cells are high efficiency cells with a substantially lower cost per reaction and are the most economical choice for standard cloning and library construction.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

• share the most useful genetic elements of standard cloning strains like DH5a, DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
• incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability.
• provide solutions for a wide range of applications at economical prices.
• Blue - white screening

Components

• SIG10 HIGH electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

Safety Information

• Storage Class Code: 10 - Combustible liquids