CLLS1003

DLD1 Cells PIK3CA +/-

human male colon (Source Disease: Colorectal adenocarcinoma)

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• product name: DLD1 Cells PIK3CA +/-,
• biological source: human male colon (Source Disease: Colorectal adenocarcinoma)
• OMIM accession no.: 171834
• storage temp.: −196°C
• Gene Information: human ... PIK3CA(5290)

Description

General description

DLD1 Cells PIK3CA +/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.

Application

Media Renewal changes two to three times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3 to 1:10

The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 3q23.3

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Also Available Separately

• DLD1 Control Cells 1 vial
• DLD1 Cells PIK3CA +/- 1 vial

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable