product name
LB253, 98062330
biological source
human blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human lymphocytes, Xeroderma pigmentosum group C, EBV-transformed
Cell Line Description
Derived from mononuclear cells from the peripheral blood of a 67 year old male. The patient was diagnosed with Xeroderma pigmentosum group C and showed spontaneous regression of melanomas. A normal level of natural killer (NK) cell activity and number, despite marked lymphopenia, was reported. The original sample was used in UV irradiation studies where UV-sensitivity of lymphocytes and fibroblasts was detected. In addition, a defect in excision repair was reported.
Application
DNA repair deficiency studies, hprt mutant analysis
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 8,12
THO1: 6,9.3
TPOX: 8
vWA: 17,19
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 8,12
THO1: 6,9.3
TPOX: 8
vWA: 17,19
Culture Medium
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml 5%
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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