biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS1
Reactivity
Anti-Thy-1, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS1 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against Thy-1 present in human brain, with small amounts in kidney. The 23.5kD a Thy-1 antigen is absent from human thymocytes and peripheral lymphocytes. Thymus, lymph node and spleen have small populations of F15-42-1-5 positive cells.
Immunogen
Purified human brain Thy-1
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 2-5x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder cells upon resuscitation
Other Notes
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