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C8093

Sigma-Aldrich

Monoclonal Anti-Connexin-43 antibody produced in mouse

clone CXN-6, ascites fluid

Synonym(s):

Mouse monoclonal antibody

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About This Item

MDL number:
MFCD00803532
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CXN-6, monoclonal

mol wt

antigen 43 kDa

contains

15 mM sodium azide

species reactivity

rat, hamster, bovine, mouse, human

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:8,000-1:16.000 using mouse whole brain extract

isotype

IgM

UniProt accession no.

P17302

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GJA1(2697) , GJA1P1(2698)
mouse ... Gja1(14609)
rat ... Gja1(24392)

General description

Monoclonal Anti-Connexin-43 (mouse IgM isotype) is derived from the CXN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Connexin 43 (Cx43) is also known as GJA1 (gap junction protein alpha 1). The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops and a C-terminal cytoplasmic tail of varying length.
The 43 kDa connexin protein (connexin-43, Cx43) belongs to the a-type (group II) subfamily of connexin proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types. For example, in the brain it is found in astrocytes, ependyma and leptomeninges but not in neurons, oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes.

Specificity

Monoclonal Anti-Connexin-43 reacts specifically with connexin-43. Reactivity has been observed with human, bovine, hamster, rat, and mouse connexin-43.

Immunogen

synthetic connexin-43 peptide (362-381).

Application

Monoclonal Anti-Connexin-43 antibody produced in mouse has been used in:
  • fluorescent immunostaining
  • western blotting
  • immunocytochemistry
  • preparation of immuno-affinity columns

Biochem/physiol Actions

Mutations in connexin 43 (Cx43) gene result in pleiotropic phenotype of oculodentodigital dysplasia. Connexin 43 participates in the multiplication and differentiation of mature T cells. It also participates in the secretion and manufacturing of cytokines. It helps to release lactate from glycolytic pancreatic ductal adenocarcinoma cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C8093-.2ML:
C8093-BULK:
C8093-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Faten Shaeib et al.
Reproductive sciences (Thousand Oaks, Calif.), 23(4), 498-507 (2015-10-16)
We investigated the ability of reactive oxygen species (ROS), such as hydrogen peroxide (H(2)O(2)), hydroxyl radical ((·)OH), and hypochlorous acid (HOCl), to overcome the defensive capacity of cumulus cells and elucidate the mechanism through which ROS differentially deteriorate oocyte quality.
Daniel J Jagger et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(48), 15851-15860 (2014-11-28)
The loss of auditory hair cells triggers repair responses within the population of nonsensory supporting cells. When hair cells are irreversibly lost from the mammalian cochlea, supporting cells expand to fill the resulting lesions in the sensory epithelium, an initial
Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123)
Axelsen LN, et al.
Journal of Molecular and Cellular Cardiology, 40(6), 790-798 (2006)
Up-regulation of gap junction in peripheral blood T lymphocytes contributes to the inflammatory response in essential hypertension
Ni X, et al.
Testing, 12(9) (2017)
Martina Maass et al.
Cell transplantation, 26(1), 157-170 (2016-08-20)
Cardiac cell replacement therapy is a promising therapy to improve cardiac function in heart failure. Persistence, structural and functional maturation, and integration of transplanted cardiomyocytes into recipients' hearts are crucial for a safe and efficient replacement of lost cells. We
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