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C8034

Sigma-Aldrich

Anti-Clathrin antibody produced in goat

whole antiserum

Synonym(s):

Anti-CHC, Anti-CHC17, Anti-CLH-17, Anti-CLTCL2, Anti-Hc, Anti-MRD56

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About This Item

MDL number:
MFCD00162235
UNSPSC Code:
12352203

biological source

goat

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

human

technique(s)

indirect immunofluorescence: 1:20 using human foreskin fibroblasts

UniProt accession no.

Q00610

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CLTC(1213)

General description

Clathrin consists of three heavy chains, along with three light chains. The weight of heavy chains varies depending on the source of clathrin. It forms a three-legged structure termed triskelion with three clathrin heavy chains (molecular weight 180,000 daltons per chain) and three non-covalently bound light chains having molecular weights of 30,000-40,000 daltons per chain. Although the term clathrin was originally used to designate the heavy chain component of triskelions, clathrin is now used to refer to the entire triskelion protein molecule.
Clathrin consists of three heavy chains, along with three light chains. The weight of heavy chains varies depending on the source of clathrin. It forms a three-legged structure, termed triskelion with three clathrin heavy chains (molecular weight 180,000 daltons per chain) and three non-covalently bound light chains having molecular weights of 30,000-40,000 daltons per chain. Although the term clathrin was originally used to designate the heavy chain component of triskelions, clathrin is now used to refer to the entire triskelion protein molecule.

Specificity

The product specifically labels the heavy chain band of clathrin by immunoblotting.

Immunogen

clathrin from bovine brain coated vesicles

Application

Anti-Clathrin antibody was used:
  • for incubating trophozites (2.105) in a study to determine the role of clathrin in endocytic process of fluid-phase pathway.
  • in indirect immunofluorescence staining.
  • for immunocytohistochemistry study of human platelets to study the colocalization of adhesive proteins with clathrin in human blood platelets.

Biochem/physiol Actions

Clathrin is the main constituent of the polygonal network that forms the layer of coated vesicles and coated pits. It is widely assumed that coated vesicles mediate the selective transfer of molecules and membrane components between specific membranous organelles within cells. It is a type of protein which plays an important function within the cell, including various types of intracellular transport processes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C8034-.2ML:
C8034-BULK:
C8034-VAR:
C8034-1ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Molecular structure, function, and dynamics of clathrin-mediated membrane traffic
Kirchhausen T, et al.
Cold Spring Harbor Perspectives in Biology, 6(5), a016725-a016725 (2014)
S A Morris et al.
The Journal of biological chemistry, 265(6), 3354-3357 (1990-02-25)
Protein kinases which co-purify with clathrin-coated vesicles are known to phosphorylate in vitro the 50-kDa subunit of the HA-II adaptor complex and upon inclusion of polylysine the beta-light chain of clathrin and polypeptides above 100 kDa. Here we relate the
M H Klinger et al.
Cell and tissue research, 279(3), 453-457 (1995-03-01)
Coated membranes and vesicles play an important role in receptor-mediated endocytosis and intracellular trafficking in various cell types, and are also present in blood platelets. Platelets take up certain proteins from the blood plasma, such as von Willebrand factor and
M Majeed et al.
Infection and immunity, 59(12), 4465-4472 (1991-12-01)
Immunofluorescence was used to examine the distribution of Chlamydia trachomatis serovars L2 and E, F-actin, and clathrin in infected McCoy and HeLa cells. After incubation at 4 degrees C, C. trachomatis serovar L2 was randomly distributed on the McCoy cell
Fernando López-Soto et al.
International journal for parasitology, 39(4), 417-426 (2008-10-14)
Entamoeba histolytica is a parasitic protozoan that produces dysentery and often reaches the liver, leading to abscess formation. Ferritin is an iron-storage protein that is mainly found in liver and spleen in mammals. The liver contains a plentiful source of
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