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C7104

Sigma-Aldrich

Anti-Cdc27 antibody,Mouse monoclonal

clone AF3.1, purified from hybridoma cell culture

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About This Item

MDL number:
MFCD01633250
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

AF3.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 97 kDa

species reactivity

mouse, Xenopus, rat, bovine, canine, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using a HeLa Cell nuclear extract

isotype

IgG2b

UniProt accession no.

P30260

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDC27(996)
mouse ... Cdc27(217232)
rat ... Cdc27(360643)

General description

Monoclonal Anti-Cdc27 (mouse IgG2b isotype) is derived from the AF3.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminal of human Cdc27, conjugated to KLH.
The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus.

Immunogen

synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.

Application

Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications:
  • Immunocytochemistry using 4% paraformaldehyde fixation and Triton X-100 permeabilization
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • Immunoprecipitation.
  • Indirect ELISA
  • Microarray
  • Western blotting / immunoblotting at a concentration of 1-2μg/mL using a HeLa Cell nuclear extract.
  • Anaphase-promoting complex/cyclosome APC/C Purification.

Biochem/physiol Actions

CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer.
The Cdc16/27/34 complex or the Anaphase-promoting complex/cyclosome (APC/C) complex functions as an ubiquitin conjugating enzyme, ubiquitinating cyclin B, and resulting in cyclin B/Cdk complex degradation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B
King R W, e al.
Cell, 81(2), 279-288 (1995)
Hui Guo et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(7), 5299-5304 (2015-02-15)
Cdc27, as a core component of anaphase-promoting complex (APC), is a cell cycle regulator, which participates in control of mitotic checkpoint and surveys the mitotic spindle to maintain chromosomal integrity. It was hypothesized that polymorphisms in cdc27 gene might contribute
Sanjay Shete et al.
Cancer research, 71(24), 7568-7575 (2011-11-01)
Gliomas, which generally have a poor prognosis, are the most common primary malignant brain tumors in adults. Recent genome-wide association studies have shown that inherited susceptibility plays a role in the development of glioma. Although first-degree relatives of patients exhibit
Leana M Topper et al.
Cell cycle (Georgetown, Tex.), 1(4), 282-292 (2002-11-14)
Cell cycle regulated protein ubiquitination and degradation within subcellular domains may be essential for the normal progression of mitosis. Cdc27 is a conserved component of an essential M-phase ubiquitin-protein ligase called the anaphase-promoting complex/cyclosome. We examined the subcellular distribution of
Munemichi Ohe et al.
Molecular biology of the cell, 21(6), 905-913 (2010-01-22)
Emi2 (also called Erp1) inhibits the anaphase-promoting complex/cyclosome (APC/C) and thereby causes metaphase II arrest in unfertilized vertebrate eggs. Both the D-box and the zinc-binding region (ZBR) of Emi2 have been implicated in APC/C inhibition. However, it is not well
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