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C-39891

Sigma-Aldrich

Hematopoietic Progenitor Expansion Medium DXF

Cytokine Mix E for 500 ml

Synonym(s):

Primary Cell Culture Media

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

aqueous solution

packaging

pkg of 1 vial

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Cytokine Mix E for HPC-Expansion Medium DXF

Application

Cytokine Mix E is a ready-to-use mixture of growth factors optionally available for the HPC Expansion Medium DXF (C-28021). The animal component-free, defined formula contains recombinant human TPO, SCF, flt-3 ligand and IL-3. The optimized formulation allows vigorous expansion of hematopoietic progenitor cells, while maintaining their primitive phenotype. Differentiation is largely inhibited.

Quality

All lots of PromoCell Hematopoietic Progenitor Media are subjected to comprehensive quality control tests using primary human mononuclear cells as well as purified hematopoietic progenitor cells from cord blood. Each lot of PromoCell HPC Expansion Medium DXF is tested for the ability to support the expansion of hematopoietic progenitor cells with a primitive phenotype. Each lot of PromoCell Hematopoietic Progenitor Medium is tested for the capacity to induce directed differentiation into hematopoietic lineages using purified CD34+ progenitor cells from cord blood. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Cytokine Mix at -20°C in the dark immediately after arrival. If stored properly, the it is stable until the expiry date stated on the label. Medium supplemented with Cytokine Mix E should be used within 2 weeks after addition of the cytokines. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C-39891:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages. 

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Protocols

In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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