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B3934

Supelco

Biuret reagent

protein detection level 150-1,000 μg/mL

Synonym(s):

Micro Lowry, Onishi & Barr Modification

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About This Item

UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.32

form

liquid

protein detection level measuring range

150-1,000 μg/mL

storage temp.

2-8°C

General description

Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm. Biuret method is less sensitive when compared to other protein estimation methods like Lowry and Folin-Ciocalteu techniques.

Application

Biuret reagent has been used for protein quantification of high-pressure thermal hydrolysis lysate(HPTH), oyster hemolymph and Clostridium cellulovorans (CBDclos) aggregates.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

B3934-BULK:
B3934-110ML-KC:
B3934-110ML:
B3934PROC:
B3934-VAR:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Proteins and Albumin
Roger LB
Laboratory Medicine, 45(1), e25?e41-e25?e41 (2014)
Production of volatile fatty acids by fermentation of waste activated sludge pre-treated in full-scale thermal hydrolysis plants
Morgan-Sagastume F, et al.
Bioresource Technology, 102(3), 3089-3097 (2011)
Bacterial inclusion bodies as potential synthetic devices for pathogen recognition and a therapeutic substance release
Talafova K, et al.
Microbial cell factories, 12(1), 16-16 (2013)
Non-lethal determination of sex and reproductive condition of Eastern oysters Crassostrea virginica Gmelin using protein profiles of hemolymph by Proteinchip textregistered and SELDI-TOF-MS technology
Li Y, et al.
Aquaculture (Amsterdam, Netherlands), 309(1-4), 258-264 (2010)
A simplified method of quantitating protein using the biuret and phenol reagents.
S T Ohnishi et al.
Analytical biochemistry, 86(1), 193-200 (1978-05-01)

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