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A8781

Sigma-Aldrich

β-Amylase from sweet potato

greener alternative

Synonym(s):

1,4-α-D-Glucan maltohydrolase

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1 VIAL
¥30,700
5 X 1 VIAL
¥116,000

¥30,700

Available to ship onMarch 31, 2025Details

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1 VIAL
¥30,700
5 X 1 VIAL
¥116,000

About This Item

CAS Number:
9000-91-3
Enzyme Commission number:
3.2.1.2 (BRENDA, IUBMB)
EC Number:
232-566-1
MDL number:
MFCD00081391
UNSPSC Code:
41106514
NACRES:
NA.56

¥30,700

Available to ship onMarch 31, 2025Details

form

powder

mol wt

~200,000

packaging

vial of 15 mg

greener alternative product score

old score: 57
new score: 1
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greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
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Greener Alternative Product

greener alternative category

Re-engineered,

storage temp.

−20°C

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General description

β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region.[1] It belongs to the glycoside hydrolase family GH14.[2]
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Application

β-Amylase from sweet potato has been used:
  • as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)[3]
  • as a standard for calibration curve preparation in size exclusion chromatography[4]
  • as a model protein for small-angle X-ray analysis (SAXs)[5]

Biochem/physiol Actions

β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends.[2] Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.[6]

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A8781-1VL:
A8781-1VL-KC:
A8781-BULK:
A8781-VAR:
A8781PROC:
A8781-5X1VL:

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Certificates of Analysis (COA)

Lot/Batch Number
0000290697
0000290697
SLCQ9616
SLCQ9616
SLCP3236

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Determination of hydrodynamic radius of proteins by size exclusion chromatography
LaVV, et al.
Bio-protocol, 7(8), 14-Jan-14-Jan (2017)
Purification of staphylococcal protein A by affinity precipitation using a reversibly soluble-insoluble polymer with human IgG as a ligand
Taniguchi, M. et al.
Journal of Fermentation and Bioengineering, 68, 32-36 (1989)
Khalilah G Reddie et al.
Molecular bioSystems, 4(6), 521-531 (2008-05-22)
Oxidation of the thiol functional group in cysteine (Cys-SH) to sulfenic (Cys-SOH), sulfinic (Cys-SO2H) and sulfonic acids (Cys-SO3H) is emerging as an important post-translational modification that can activate or deactivate the function of many proteins. Changes in thiol oxidation state
Luca Costa et al.
BMC structural biology, 16(1), 18-18 (2016-10-30)
Synchrotron radiation facilities are pillars of modern structural biology. Small-Angle X-ray scattering performed at synchrotron sources is often used to characterize the shape of biological macromolecules. A major challenge with high-energy X-ray beam on such macromolecules is the perturbation of
Optimization of the immobilization of sweet potato amylase using glutaraldehyde-agarose support. Characterization of the immobilized enzyme
Tavano OL, et al.
Process. Biochem., 48(7), 1054-1058 (2013)
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Protocols

Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.

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