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A8311

Sigma-Aldrich

Antifoam 204

aqueous emulsion for bacterial and mammalian systems

Synonym(s):

organic antifoam

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About This Item

MDL number:
MFCD00130523
UNSPSC Code:
12161901
NACRES:
NA.85

Quality Level

200

form

emulsion (aqueous)

concentration

100% (organic)

density

1.01 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

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General description

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.
Antifoam 204 has been used:
  • in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain
  • in fed-batch fermentation
  • to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth
  • in the batch fermentation with mineral medium

Components

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

Other Notes

Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly.

Preparation Note

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.

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Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A8311-PH:
A8311-BULK:
A8311-50ML-PW:
A8311PROC:
A8311-50ML:
A8311-VAR:
A8311-10ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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USP

USP

1612652

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