Skip to Content
Merck
All Photos(1)

Documents

Safety Information

A7080

Sigma-Aldrich

Amphiregulin human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

Synonym(s):

AREG, CRDGF, SDGF, AR

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
117147-70-3
MDL number:
MFCD00282118
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

Quality Level

100

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

10-100 ng/mL ED50/EC50

mol wt

~11 kDa

packaging

pkg of 1 mg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P15514

storage temp.

−20°C

Gene Information

human ... AREG(374)

Related Categories

Biochem/physiol Actions

Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.

Components

Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.

Caution

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.

Preparation Note

It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.

Analysis Note

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A7080-.1MG-PW:
A7080-BULK:
A7080-.1MG:
A7080-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Dalia Burzyn et al.
Cell, 155(6), 1282-1295 (2013-12-10)
Long recognized to be potent suppressors of immune responses, Foxp3(+)CD4(+) regulatory T (Treg) cells are being rediscovered as regulators of nonimmunological processes. We describe a phenotypically and functionally distinct population of Treg cells that rapidly accumulated in the acutely injured
Jan-Eric Turner et al.
The Journal of experimental medicine, 210(13), 2951-2965 (2013-11-20)
IL-9 fate reporter mice established type 2 innate lymphoid cells (ILC2s) as major producers of this cytokine in vivo. Here we focus on the role of IL-9 and ILC2s during the lung stage of infection with Nippostrongylus brasiliensis, which results
Khampoun Sayasith et al.
General and comparative endocrinology, 180, 39-47 (2012-11-28)
Little is known about the expression and regulation of epiregulin (EREG) and amphiregulin (AREG) in ovarian follicles of large monoovulatory animal species. To characterize the gonadotropin-dependent regulation of EREG and AREG mRNAs in equine follicles prior to ovulation, extracts were
L Damstrup et al.
British journal of cancer, 80(7), 1012-1019 (1999-06-11)
Colonic enterocytes, like many epithelial cells in vivo, are polarized with functionally distinct apical and basolateral membrane domains. The aims of this study were to characterize the endogenous epidermal growth factor (EGF)-like ligands expressed in two polarizing colon cancer cell
Renuka Subramaniam et al.
The Journal of infectious diseases, 209(11), 1827-1836 (2013-12-25)
Seasonal and especially pandemic influenza predispose patients to secondary bacterial pneumonias, which are a major cause of deaths and morbidity. Staphylococcus aureus is a particularly common and deadly form of post-influenza pneumonia, and increasing staphylococcal drug resistance makes the development
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service