A6781

Acetate Kinase from Bacillus stearothermophilus

lyophilized powder, 400-1,200 units/mg solid

• Synonym(s): ATP:Acetate phosphotransferase, Acetate Kinase Bacillus stearothermophilus
• CAS Number: 9027-42-3
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 2.7.2.1 (BRENDA, IUBMB)
• MDL number: MFCD00130330
• Specific activity: 400-1,200 units/mg solid
• Biological source: bacterial (Bacillus stearothermophilus)

Properties

• biological source: bacterial (Bacillus stearothermophilus)
• Quality Segment: 200
• form: lyophilized powder
• specific activity: 400-1,200 units/mg solid
• storage temp.: 2-8°C

Description

Application

Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. ^[32P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [³²P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.

Biochem/physiol Actions

Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 % α-helix and 43 % unordered structure.

Involved in the metabolism of propanoate, pyruvate and taurine.

Physical form

Contains Tris-HCl buffer.

Other Notes

One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30 °C.

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 11 - Combustible Solids