A6680

N-Acetylneuraminic Acid Aldolase from microorganisms

lyophilized powder, ≥20 units/mg protein (biuret)

• Synonym(s): N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase
• CAS Number: 9027-60-5
• Enzyme Commission number: 4.1.3.3 (BRENDA, IUBMB)
• Beilstein: 2697172
• MDL number: MFCD00131656
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• form: lyophilized powder
• specific activity: ≥20 units/mg protein (biuret)
• mol wt: ~98 kDa
• storage temp.: −20°C

Description

Application

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.

Used in the Sialic Acid Quantification Kit, SIALIC-Q

Physical properties

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾³M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg⁺⁺, Ag⁺
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)

Unit Definition

One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C.

Physical form

Lyophilized powder containing mannitol and EDTA

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves