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A5545

Sigma-Aldrich

Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Guinea pig IgG (whole molecule)−HRP

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About This Item

MDL number:
MFCD00162368
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

guinea pig

technique(s)

direct ELISA: 1:50,000-1:80,000
dot blot: 1:50,000-1:100,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. It has two subclasses of IgG- IgG1 and IgG2.

Specificity

Rabbit anti-guinea pig IgG (whole molecule)-peroxidase antibody binds to guinea pig IgG.

Immunogen

guinea pig IgG

Application

Anti-Guinea Pig IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in diphtheria serology enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

IgGs are glycoprotein antibodies that modulate several immune responses. Guinea pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-guinea pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) is the major class and most abundant immunoglobulin of immune system released by plasma cells. IgG mediate immune response with respect to the type of antigen and is found in blood or extracellular fluid. IgG defends against bacterial and viral infections. Maternal IgG crosses from mother to fetus through the placental barrier by pinocytosis to provide passive immunity for the neonate and protect against infections.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG1 helps in complement fixation through alternate pathway and IgG2 fixes complement by conventional mechanism. It mainly helps in immune defense. Guinea Pig is a highly preferred laboratory animal to study the local and anaphylactic reactions.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Storage and Stability

Store at -20 °C for long term use. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A5545-1ML:
A5545-BULK:
A5545-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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