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A5170

Sigma-Aldrich

Anti-Avidin antibody produced in rabbit

whole antiserum

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About This Item

MDL number:
MFCD00162144
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

technique(s)

immunoelectrophoresis: suitable
indirect ELISA: 1:100,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties. Avidin from corn has low molecular weight than chicken egg-derived avidin. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Immunogen

avidin

Application

Anti-Avidin antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in preparation of antibody-Au nanoparticles.
The presence of Scavidin in BT4C glioma cells was assessed by western blot using a rabbit anti-avidin antibody as the primary at a dilution of 1:4000. Lehtolainen, P. (2002) Cloning and Characterization of Scavidin, a Fusion Protein for the Targeted Delivery of Biotinylated Molecules. J. Biol. Chem. 277:8545-8550.

Biochem/physiol Actions

Avidin binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Physical form

Supplied as a liquid containing 15mM sodium azide as preservative

Preparation Note

treated to remove lipoproteins

Analysis Note

This antisera is evaluated for performance and specificity by immunodiffusion and immunoelectrophoresis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A5170-1ML:
A5170-BULK:
A5170-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Development of transgenic wheat (Triticum aestivum L.) expressing avidin gene conferring resistance to stored product insects
Abouseadaa HH, et al.
BMC plant biology, 15(1), 1-8 (2015)
Coomassie Brilliant Dyes as Surface-Enhanced Raman Scattering Probes for Protein-Ligand Recognitions
Han, Xiao X. and others
Analytical Chemistry, 82(10), 4102-4106 (2010)
Commercial plant-produced recombinant avidin
Commercial Plant-Produced Recombinant Protein Products, 15-25 (2014)
Plant molecular farming: systems and products
Horn ME, et al.
Plant Cell Reports, 22(10), 711-720 (2004)
Heba H Abouseadaa et al.
BMC plant biology, 15, 183-183 (2015-07-22)
Wheat is considered the most important cereal crop all over the world. The wheat weevil Sitophilus granarius is a serious insect pests in much of the wheat growing area worldwide and is responsible for significant loss of yield. Avidin proteins
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