immunoprecipitation (IP): 1-2 μg/mL using whole extract of HEK 293 cells indirect immunofluorescence: 2-5 μg/mL
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Adenovirus E1A gene modulates gene expression by associating with transcription factors in cells. Studies have reported that E1A regulates the stimulation of ICAM-1 through inflammatory signals in pulmonary epithelial cells. E1A has also been implicated in chronic obstructive pulmonary disease (COPD). Furthermore, E1A is known to mediate tumor suppression by rendering epithelial cells sensitive to anoikis. Mouse Monoclonal Anti-Adenovirus 2 E1A antibody binds to adenovirus 2 and 5 E1A (3 bands at 30-50kDa by SDS-PAGE), as well as E1A associated proteins.
Immunogen
full length recombinant adenovirus 2 E1A protein.
Application
Monoclonal Anti-Adenovirus 2 E1A can be used for indirect immunofluorescence (2-5μg/mL) and immunoprecipitation (1-2μg/mL, using whole extract of HEK 293 cells).
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
American journal of respiratory cell and molecular biology, 16(1), 23-30 (1997-01-01)
Previous studies from our laboratory demonstrated that adenovirus E1A DNA and proteins are detected in lungs of patients with chronic obstructive pulmonary disease (COPD). Since adenovirus E1A gene products are known to regulate the expression of many genes by interacting
Tumor suppressor genes such as Rb and p53 usually kill tumor cells when overexpressed ectopically. This is a consequence of their normal cell cycle regulatory functions. By contrast, the E1a gene of adenovirus, a common cold virus, converts tumor cells
Experimental and therapeutic medicine, 15(6), 5394-5402 (2018-05-31)
Viral vectors represent a potential strategy for the treatment of human malignant tumors. Currently, recombinant adenovirus vectors are commonly used as gene therapy vehicles, as it possesses a proven safety profile in normal human cells. The recombinant adenovirus system has
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