A4362

Activin A active human

Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)

• Synonym(s): Erythroid differentiation protein, Inhibin beta A chain; Activin beta-A chain
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in Nicotiana
• Assay: >97% (SDS-PAGE)
• form: lyophilized
• potency: ≤5 ng/mL ED₅₀
• mol wt: 27.4 kDa (disulfide-linked homodimers of two βA chains, each containing 116 amino residues)
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
• UniProt accession no.: P08476
• storage temp.: −20°C
• Gene Information: human ... INHBA(3624)

Description

Amino Acid Sequence

HisHisHisHisHisHisGlyLeuGluCysAspGlyLysValAsnIleCysCysLysLysGlnPhePheValSerPheLysAspIleGlyTrpAsnAspTrpIleIleAlaProSerGlyTyrHisAlaAsnTyrCysGluGlyGluCysProSerHisIleAlaGlyThrSerGlySerSerLeuSerPheHisSerThrValIleAsnHisTyrArgMetArgGlyHisSerProPheAlaAsnLeuLysSerCysCysValProThrLysLeuArgProMetSerMetLeuTyrTyrAspAspGlyGlnAsnIleIleLysLysAspIleGlnAsnMetIleValGluGluCysGlyCysSer

General description

Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβ family. Mature Activin A has two 116 amino acids residues βA subunits (βA-βA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells.
As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.

Physical form

Lyophilized from a Tris HCl 0.05 M buffer at pH 7.4

Reconstitution

Lyophilized protein should be reconstituted in water to a concentration of 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.

Analysis Note

The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation.
ED₅₀: ≤ 5 ng/mL

Other Notes

Extinction Coefficient: E^0.1% = 1.27 (A ₂₈₀ nm)
p.I: 7.27

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable