A3125

8-Anilino-1-naphthalenesulfonic acid ammonium salt

~95%, crystalline

• Synonym(s): 1,8-ANS NH₄, ANSA, Ammonium 8-anilino-1-naphthalenesulfonate, N-Phenyl peri acid
• Linear Formula: C₁₆H₁₃NO₃S · NH₃
• CAS Number: 28836-03-5
• Molecular Weight: 316.37
• Beilstein: 3581235
• EC Number: 249-265-6
• MDL number: MFCD00012560
• UNSPSC Code: 12352200

Properties

• Assay: ~95%
• form: crystalline
• color: dark green
• solubility: NaOH: 1 N; H₂O: soluble; methanol: soluble
• fluorescence: λ_ex 388 nm; λ_em 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA
• SMILES string: N.OS(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12
• InChI: 1S/C16H13NO3S.H3N/c18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;/h1-11,17H,(H,18,19,20);1H3
• InChI key: IPBNQYLKHUNLQE-UHFFFAOYSA-N

Description

Application

ANS forms an inclusion complex with cyclodextrin. Such model systems are useful to mimic biological recognition and can be studied by measuring the change in fluorescence of free-ANS to complexed-ANS. When ANS enters the hydrophobic core of cyclodestrin, it′s fluorescence increases . Utilized in the reagent phase of a sodium-selective fiber-optic sensor. The reagent phase also contains a copper(II) polyelectrolyte, which binds to ANSA in the absence of sodium and quenches the fluorescence. In the presence of sodium, ANSA forms a cationic complex creating ion-pairs, causing it to fluoresce . ANS is often incorporated into di-block polymers and can be released by changes in the local environment (i.e., temperature, pH, etc.) . ANS is commonly used as a fluorescence probe to investigate molecular assemblies of surfactants and amphiphilic polymers because a blue shift of the emission maximum indicates the fluorophore is located in less polar media . Fluorescent probe for protein studies using methodologies such as steady-state and dynamic fluorescence measurements .

Fluorescent probe for protein studies.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves