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A2831

Sigma-Aldrich

Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Anti-Dinitrophenyl, Monoclonal Anti-DNP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SPE-7, monoclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:25,000 using DNP-BSA
dot blot: 1:180,000 (chemiluminescence)

isotype

IgE

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.

Immunogen

DNP-KLH conjugate

Application

Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 50% glycerol and 15 mM Sodium azide

Analysis Note

Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A2831-.5ML:
A2831-VAR:
A2831-1ML:
A2831-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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